io

IO Parallel Mini Fermenters/Bioreactors

IO is a high-throughput fermentation system for easy control by beginners and experienced user alike. It is ideal for microbial, mammalian, plant, human and anaerobic cells.

  • The most important fermentation parameters like cell density concentration, pH and dissolved oxygen can be monitored online.
  • The system is designed for defined mass transfer conditions (i.e. no oxygen limitation).
  • With total volumes 200ml, 400ml, 600ml, 1000ml and 2 different ratio H/D is the ideal device for innovative parallel process development.
  • IO is an ideal partner for applications in: Cell and strain screening, Protein expression and kinetics, Enzyme activity tests, Fermentation parameter optimization, Synthetic biology.
  • LEDA Sampling System:
  • Sterile single use sampling system up to 180 sterile sampling per batch.
  • Needlefree connector is designed to reduce the risk of contamination during sampling
  • Various controller and hardware configurations enable aeration strategies using air, oxygen, nitrogen or a mixture of these to enrich the air.
  • The mass-flow controller allows the exact flow rate control of individual gases.
Vessel
Solaris  Code IO 200 IO 400 IO 600 IO 1000
Total Volume (ml) 200 400 600 1000
Ratio H/D 1.5 2.5 1.5 2.5
Min. Working Volume (ml) 100 200 300 500
Max. Working Volume (ml) 150 300 450 750
Max Temp. 70°C
Max Operating pressure 0.8 bar (g)
n. 8 Ports n.3 DN12 ports  (sensors, multifeed) – n.2 DN8 ports  (gas in sparger, harvest/sampling) – n.3 DN9 (gas out, temperature probe, antifoam probe)
Length 120 120 120 120
Dimensions for autoclave (with Condenser)
Height (mm) 190 260 260 450
Diameter (mm 150 150 200 200
Materials Borosilicate glass and  AISI 316 L
Stirring
Drive Brushless Motor, Direct Assembly , 1-2600 rpm
Power 100 W
Impellers Select from: Rushton impellers, Marine Impellers, Pitched blade
Thermoregulation
Control PID Control – Accuracy 0,1 °C – Peltier Cell
Gas Control & Gas Mixing
Sparger and overlay Gas Control TMFC
Sparger type Microholes provided with 0,2 µm filter
Exhaust 0,2 µm filter
Peristaltic Pumps
up to 4 Watson Marlow 400F/A,  speed 13 – 290  rpm, volumetric flow 0,03-30 ml/min, application assignable from  software
Controller
PCS from  1 to 24 units    –  Dimensions  Height:  260  mm   Largeness: 260  mm   Depth: 260  mm
HMI with Leonardo software 23″ for single units, 27″ for multi systems (parallel)
Temperature
Sensor PT100
Control system Measuring resident in Leonardo 2.0 software
Control range 0 – 150°C
pH
Sensor Digital Hamilton sensor
Control system Measuring resident in Leonardo 2.0 software
Control range 0 – 14
Operation temperature 0 – 130°C
Pressure range 0 – 6 bar
Actuator Cascade to peristlatic pumps for the addition of acid/base solutions or gas (CO2)
dO2
Sensor Digital Digital Optical Hamilton sensor
Control system Measuring resident in Leonardo 2.0 software
Control range 0,05 – 300% air saturation
Operation temperature -10 – 130°C
Pressure range 0 – 12 bar
Actuator Cascade to RPM, Gas Control, feedings,etc
dCO2
Sensor Mettler Toledo sensor 
Control system Measuring resident in Leonardo 2.0 software
Control range 0,00-200% saturation
Operation temperature -20.0-150°C
Pressure range 0 – 4 bar
Turbidity
Sensor Mettler Toledo sensor
Control system Measuring resident in Leonardo 2.0 software
Measuring range 0-250 g/L, 10-4000 FTU
Cell density
Sensor Hamilton-Fogale sensor
Control system Measuring resident in Leonardo 2.0 software
Option 1 Total cell density (Two ranges: 10^5 to 10^8 mammalian cells/ml – 0.5 to 300 g/L dry weight)
Option 2 Viable cell density (Two ranges: 5×10^5 to 8×10^8 mammalian cells/ml – 5 to 200 g/L dry weight)
Redox (ORP)
Sensor  Digital Hamilton sensor 
Control system Measuring resident in Leonardo 2.0 software
Control range ±2000 mV
Operation temperature – 10 -130°C
Pressure range ≤ 6 bar
Conductivity
Sensor  Digital Hamilton sensor 
Control system Measuring resident in Leonardo 2.0 software
Control range 1 – 3000 μS/cm
Operation temperature 0 -130°C
Pressure range 0 – 20 bar
Weight
Sensor n.3 load cells
Control Measuring resident in Leonardo 2.0 software
Antifoam/Level
Sensor Solaris sensor
Control Measuring resident in Leonardo 2.0 software